Application
Western blot analysis of UCKL1 expression in 293 whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue showing cytoplasmic localization.| Product Name | UCKL1 Antibody (E-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of UCKL1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | UCKL1 (uridine-cytidine kinase 1-like 1), also known as UCK1L, URKL1 or F538, is a ubiquitously expressed 548 amino acid member of the uridine kinase family. Localized to the cytoplasm and translocated to the nucleus via interaction with EBV EBNA-3A (an Epstein-Barr nuclear antigen), UCKL1 is thought to participate in pyrimidine metabolism by accumulating UTP and CTP, both of which are needed for cell proliferation and blast transformation. UCKL1 contains an N-terminal ATP/GTP-binding site and, once relocated to the nucleus, becomes part of the ATP-dependent ribonucleotide salvage pathway that catalytically converts UTP and CTP to UMP and CMP, respectively. In addition, UCKL1 functions as a substrate for the E3 ligase NKLAM, thereby causing the ubiquitin-mediated degradation of UCKL1. Three isoforms of UCKL1 are expressed due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of UCKL1 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of UCKL1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 54963 |
|---|
Application
Western blot analysis of UCKL1 expression in 293 whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue showing cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
