Application
Western blot analysis of DARPP-32 expression in mouse brain tissue extract.Application
Western blot analysis of DARPP-32 expression in non-transfected: (A) and mouse DARPP-32 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of DARPP-32 expression in MCP-5 whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tumor showing cytoplasmic localization.Application
Immunofluorescence staining of methanol-fixed SK-N-SH cells showing cytoplasmic localization.Application
Western blot analysis of rat recombinant DARPP-32.| Product Name | DARPP-32 Antibody (H-62) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 134-195 of DARPP-32 (dopamine and adenosine 3',5'-monophosphate-regulated phosphoprotein of 32 kDa) of bovine origin |
| Modification Notes | Dopaminergic signaling pathways, which are essential for multiple brain functions, are abnormal in several neurological disorders, such as schizophrenia, Parkinson’s disease and drug abuse. DARPP-32 (for dopamine and adenosine 3’,5’-monophosphate-regulated phosphoprotein) is abundant in neurons that receive dopaminergic input. Activation of PKA and the consequent phosphorylation of DARPP-32 on threonine occurs in response to dopamine acting upon D1-like receptors. Dopamine interaction with D2-like receptors results in the inhibition of PKA activation, the activation of protein phosphatase 2B and the consequent dephosphorylation of DARPP-32. Neurotransmitters other than dopamine may also be able to stimulate the phosphorylation or dephosphorylation of DARPP-32. Phosphorylated DARPP-32 is a potent inhibitor of PP-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of DARPP-32 of human, bovine and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 134-195 of DARPP-32 (dopamine and adenosine 3' 5'-monophosphate-regulated phosphoprotein of 32 kDa) of bovine origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 134-195 of DARPP-32 (dopamine and adenosine 3',5'-monophosphate-regulated phosphoprotein of 32 kDa) of bovine origin |
| Entrez Gene | 84152 |
|---|
Application
Western blot analysis of DARPP-32 expression in mouse brain tissue extract.
Application
Western blot analysis of DARPP-32 expression in non-transfected: (A) and mouse DARPP-32 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of DARPP-32 expression in MCP-5 whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tumor showing cytoplasmic localization.
Application
Immunofluorescence staining of methanol-fixed SK-N-SH cells showing cytoplasmic localization.
Application
Western blot analysis of rat recombinant DARPP-32.| Application Notes | recommended for detection of DARPP-32 of human, bovine and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
