Application
Western blot analysis of MFT expression in Hep G2 whole cell lysate.| Product Name | MFT Antibody (B-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of MFT of mouse, rat and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Modification Notes | Folate is an essential vitamin that must be obtained from food intake through intestinal absorption in mammals. Folate and reduced folic acid derivatives bind to the folate receptor (FR) family, which mediates the endocytosis of 5-methyltetrahydofolate into the cell. MFT (Mitochondrial folate transporter/carrier), also known as Solute carrier family 25 member 32 (SLC25A32), is a 315 amino acid multi-pass membrane protein that regulates the influx of folate into the mitochondria. MFT also functions to complement glycine auxotrophy. Mutations in the gene encoding MFT may be involoved in certain cases of multiple acyl-CoA dehydrogenase deficiency (MADD), in which there is no mutation in EFTA or EFTB genes. MADD is a autosomal recessively inherited disorder in which fatty acid, amino acid and choline metabolism is disrupted, leading to a clinical manisfestation of hypoglycemia, hypotonia, hepatomegaly, metabolic acidosis and dysplastic kidneys. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of MFT of mouse rat and human origin by WB IP and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of MFT of mouse, rat and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 81034 |
|---|
Application
Western blot analysis of MFT expression in Hep G2 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
