Application
Western blot analysis of ZFP1 expression in NIH/3T3 nuclear extract (A) and rat brain tissue extract (B).Application
Western blot analysis of ZFP1 expression in Jurkat whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human intestine tissue showing nuclear and cytoplasmic localization.Application
Western blot analysis of ZFP1 expression in HeLa (A) and NIH/3T3 (B) nuclear extracts.| Product Name | ZFP1 Antibody (T-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of ZFP1 of mouse and human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krüppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. ZFP1 (zinc finger protein 1), also known as ZNF475, is a 407 amino acid protein that contains one KRAB domain and eight C2H2-type zinc fingers. Localizes to the nucleus, ZFP1 exists as multiple alternatively spliced isoforms and is thought to play a role in transcriptional regulation events. The gene encoding ZFP1 maps to human chromosome 16, which encodes over 900 genes and comprises nearly 3% of the human genome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of ZFP1 of mouse and human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of ZFP1 of mouse and human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 162239 |
|---|
Application
Western blot analysis of ZFP1 expression in NIH/3T3 nuclear extract (A) and rat brain tissue extract (B).
Application
Western blot analysis of ZFP1 expression in Jurkat whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human intestine tissue showing nuclear and cytoplasmic localization.
Application
Western blot analysis of ZFP1 expression in HeLa (A) and NIH/3T3 (B) nuclear extracts.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
