Application
Western blot analysis of PARP-6 expression in Hep G2 whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human intestine tissue showing cytoplasmic localization.Application
Western blot analysis of PARP-6 expression in Jurkat whole cell lysate.| Product Name | PARP-6 Antibody (B-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of PARP-6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Modification Notes | Poly(ADP-ribosylation) is a method of DNA damage-dependent posttranslational modification that helps to rescue injured proliferating cells from cell death. The PARP (poly(ADP-ribose) polymerase) proteins comprise a superfamily of enzymes that functionally modify histones and other nuclear proteins, thereby preventing cell death. PARPs use NAD+ as a substrate to catalytically transfer ADP-ribose residues onto protein acceptors; a process that, when repeated multiple times, leads to the formation of poly(ADPribose) chains on the protein. The presence of these chains alters the function of the target protein and promotes cell survival. PARP proteins are implicated in a variety of diseases, including cancer, neurodegenerative and inflammatory disorders. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of PARP-6 of mouse rat and human origin by WB IP IF IHC(P) and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of PARP-6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 56965 |
|---|
Application
Western blot analysis of PARP-6 expression in Hep G2 whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human intestine tissue showing cytoplasmic localization.
Application
Western blot analysis of PARP-6 expression in Jurkat whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
