Application
Western blot analysis of SLIRP expression in K-562 nuclear extract.Application
Western blot analysis of SLIRP expression in Hep G2 whole cell lysate.| Product Name | SLIRP Antibody (G-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of SLIRP of human origin by WB, IP and ELISA |
| Modification Notes | SLIRP (SRA stem-loop-interacting RNA-binding protein), also known as DC50 or C14orf156, is a 109 amino acid mitochondrial protein that localizes to the nucleus and is ubiquitously expressed, with highest levels in heart, liver, skeletal muscle and testis. Containing a single RRM (RNA recognition motif) domain, SLIRP acts as a nuclear receptor corepressor and binds the STR7 loop of SRA RNA. In addition to repressing the SRA-mediated nuclear receptor coactivation, SLIRP is also able to repress glucocorticoid (GR), androgen (AR), thyroid (TR) and VDR-mediated transactivation. It is suggested that SLIRP may regulate mitochondrial function and augment the effect of Tamoxifen, a drug that acts as an antagonist of the estrogen receptor and inhibits receptor activity. The gene encoding SLIRP is located on human chromsome 14, which houses over 700 genes and comprises nearly 3.5% of the human genome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of SLIRP of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of SLIRP of human origin by WB, IP and ELISA |
| Entrez Gene | 81892 |
|---|
Application
Western blot analysis of SLIRP expression in K-562 nuclear extract.
Application
Western blot analysis of SLIRP expression in Hep G2 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
