Application
Western blot analysis of BRD4 expression in human fetal thymus tissue extract.| Product Name | BRD4 Antibody (P-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of BRD4 of mouse, rat and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Modification Notes | Brd4 belongs to the BET family, a group of structurally related proteins containing two bromodomains. Through these two domains, Brd4 associates with mitotic chromosomes and its expression correlates with cell growth. Expression of Brd4 inhibits cell cycle progression from G(1) to S, due to binding to the largest subunit of replication factor C (RFC) to prevent DNA elongation. Altered Brd4 function correlates with poorly differentiated carcinoma, with aggresive phenotype and a highly lethal outcome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of BRD4 of mouse rat and human origin by WB IP and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of BRD4 of mouse, rat and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 23476 |
|---|
Application
Western blot analysis of BRD4 expression in human fetal thymus tissue extract.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
