Application
Western blot analysis of human Pumilio 2 transfected 293T whole cell lysate.| Product Name | Pumilio 2 Antibody (P-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Pumilio 2 of mouse, rat and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Modification Notes | Pumilio 2 is a sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding mRNA targets. It supports proliferation and self-renewal of stem cells by regulating the translation of key transcripts. The Pumilio gene encodes proteins that are required for development of germ stem cells in one or both sexes. The Pumilio protein interacts with the human Nanos1 protein and this interaction may play a conserved role in germ cell development. Pumilio 2 is highly expressed in testis and ovary and at lower levels in brain, heart, kidney, liver, muscle, placenta, intestine and stomach. It is also expressed in stem cells, germ cells and in most fetal tissues. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Pumilio 2 of mouse rat and human origin by WB IP and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of Pumilio 2 of mouse, rat and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 23369 |
|---|
Application
Western blot analysis of human Pumilio 2 transfected 293T whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
