Application
Western blot analysis of Ferrochelatase expression in Jurkat whole cell lysate.| Product Name | Ferrochelatase Antibody (D-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Ferrochelatase of mouse, rat and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Modification Notes | Ferrochelatase, also designated Heme synthetase or protoheme ferro-lyase, is the terminal enzyme of protoheme biosynthesis that catalyzes the ferrous form of iron insertion into protoporphyrin IX. Mature ferrochelatase is a homodimeric, mitochondrial membrane-associated protein translated downstream of an N-terminal 54-amino acid transit peptide. Ferrochelatase contains two nitric oxide (NO)-sensitive clusters and coordinated 2FE-2S clusters which may potentially serve as a nitric oxide sensor. Defects in the gene encoding the Ferrochelatase enzyme, FECH, cause erythropoietic protoporhyria (EPP), which is a dominantly inherited disease of porphyrin metabolism characterized by photosensitivity and hepatobiliary disease. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ferrochelatase of mouse rat and human origin by WB IP and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of Ferrochelatase of mouse, rat and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 2235 |
|---|
Application
Western blot analysis of Ferrochelatase expression in Jurkat whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
