Application
Western blot analysis of Rictor expression in Ramos whole cell lysate.| Product Name | Rictor Antibody (H-278) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against amino acids 20-297 mapping near the N-terminus of Rictor of human origin |
| Modification Notes | FRAP is a large protein kinase that is the mammalian target of rapamycin, an immunosuppressant that blocks vessel restenosis and also has potential anticancer applications. Rapamycin-insensitive companion of FRAP, also designated Rictor, shares homology with pianissimo from D. discoidieum, STE20p from S. pombe, and AVO3p from S. cerevisiae. Rictor forms a complex with FRAP, which is important in cell growth regulation as it integrates growth factor and nutrient derived signals. The Rictor-FRAP complex plays a role in PKCå phosphorylation, directly phosphorylates Akt/PKB on Ser473 in vitro and facilitates Thr308 phosphorylation by PDK1. It also may function as a drug target in tumors that have lost expression of PTEN, a tumor suppressor that opposes activation of Akt/PKB. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Rictor of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against amino acids 20-297 mapping near the N-terminus of Rictor of human origin |
|---|---|
| Tissue Specificity | raised against amino acids 20-297 mapping near the N-terminus of Rictor of human origin |
| Entrez Gene | 253260 |
|---|
Application
Western blot analysis of Rictor expression in Ramos whole cell lysate.| Application Notes | recommended for detection of Rictor of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
