Application
Western blot analysis of ABT1 expression in human fetal heart tissue extract.| Product Name | ABT1 Antibody (D-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of ABT1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | ABT1 (activator of basal transcription 1) is a nuclear protein that associates with the TATA-binding protein (TBP) and enhances basal transcription activity of class II promoters (1,2). ABT1 associates with TBP in HeLa nuclear extracts in vitro (1). Another protein, designated ERF, is a member of the ETS family of transcription factors (3). The members of the ETS family are grouped because they share a highly conserved DNA binding domain (3). These factors are involved in growth factor pathways and regulate both proliferation and differentiation (3). ERF (Ets2 Repressor Factor) is a ubiquitously expressed ets-domain protein that exhibits strong transcriptional repressor activity, suppresses ets-induced transformation and is regulated by MAPK phosphorylation (4). ERF transcription may be regulated by ets-domain proteins (4). Additionally, modulation of ERF activity is involved in the transcriptional regulation of genes activated during entry into G1 phase (5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of ABT1 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of ABT1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 29777 |
|---|
Application
Western blot analysis of ABT1 expression in human fetal heart tissue extract.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
