Application
Western blot analysis of GRSF-1 expression in Hep G2 whole cell lysate.| Product Name | GRSF-1 Antibody (T-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of GRSF-1 of human origin by WB, IP and ELISA |
| Modification Notes | GRSF-1 (G-rich RNA sequence binding factor 1) is a 480 amino acid cytoplasmic protein that contains three RRM (RNA recognition motif) domains and two auxiliary domains. Considered a cellular protein, GRSF-1 binds to RNAs containing the G-rich element. It is suggested that GRSF-1 selectively recruits cellular and viral mRNAs containing 5' UTR GRSF-1 binding sites to polyribosomes, which is mediated through interactions with cellular proteins. GRSF-1 is encoded by a gene located on human chromosome 4, which encodes nearly 6% of the human genome and has the largest gene deserts (regions of the genome with no protein encoding genes) of all of the human chromosomes. Defects in some of the genes located on chromosome 4 are associated with Huntington's disease, Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of GRSF-1 of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of GRSF-1 of human origin by WB, IP and ELISA |
| Entrez Gene | 2926 |
|---|
Application
Western blot analysis of GRSF-1 expression in Hep G2 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
