Application
Western blot analysis of ZNF76 expression in HS 181.Tes (A), K-562 (B) and MEG-01 (C) whole cell lysates.| Product Name | ZNF76 Antibody (H-100) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 364-463 mapping within an internal region of ZNF76 of human origin |
| Modification Notes | ZNF76, also known as ZNF523 or Zfp523, is a transcriptional repressor expressed in the testis. It is the human homolog of the Xenopus Staf protein (selenocysteine tRNA gene transcription-activating factor) known to regulate the genes encoding small nuclear RNA and selenocysteine tRNA. ZNF76 localizes to the nucleus and exerts an inhibitory function on p53-mediated transactivation. ZNF76 specifically targets TFIID (TATA-binding protein). The interaction with TFIID occurs through both its N and C termini. The transcriptional repression activity of ZNF76 is predominantly regulated by lysine modifications, acetylation and sumoylation. ZNF76 is sumoylated by PIAS 1 and is acetylated by p300. Acetylation leads to the loss of sumoylation and a weakened TFIID interaction. ZNF76 can be deacetylated by HDAC1. In addition to lysine modifications, ZNF76 activity is also controlled by splice variants. Two isoforms exist due to alternative splicing. These isoforms vary in their ability to interact with TFIID. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ZNF76 of human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 364-463 mapping within an internal region of ZNF76 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 364-463 mapping within an internal region of ZNF76 of human origin |
Application
Western blot analysis of ZNF76 expression in HS 181.Tes (A), K-562 (B) and MEG-01 (C) whole cell lysates.| Application Notes | recommended for detection of ZNF76 of human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
