Application
Western blot analysis of nm23-H4 expression in non-transfected: (A) and human nm23-H4 transfected: (B) 293 whole cell lysates.| Product Name | nm23-H4 Antibody (H-53) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 54-106 mapping within an internal region of nm23-H4 of human origin |
| Modification Notes | The nm23 gene (Metastasis inhibition factor nm23), a potential suppressor of metastasis, is expressed at a much lower level in highly metastatic cells than in cells with lower metastatic potential. Based on sequence analysis, nm23, also designated nucleoside diphosphate kinase A (NDK A) or Tumor metastatic process-associated protein, appears to be highly related to nucleotide diphosphate kinases (NDP). NDP kinases A and B are identical to two isotypes of human nm23 homologs, nm23-H1 and nm23-H2, respectively. nm23-H2 is also identical in sequence to PuF, a transcription factor that binds to nuclease-hypersensitive elements at positions 142 to 115 of the human c-Myc promoter. nm23-H3 and nm23-H4 are important for the synthesis of nucleoside triphosphates and may play a role in apoptosis induction and hematopoiesis. nm23-H4 localizes to the mitochondrial intermembrane space and is widely expressed, with higher levels detected in prostate, heart, liver, small intestine, and skeletal muscle tissues. Low amounts of nm23-H4 are observed in the brain and in blood leukocytes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of nm23-H4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 54-106 mapping within an internal region of nm23-H4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 54-106 mapping within an internal region of nm23-H4 of human origin |
| Entrez Gene | 4833 |
|---|
Application
Western blot analysis of nm23-H4 expression in non-transfected: (A) and human nm23-H4 transfected: (B) 293 whole cell lysates.| Application Notes | recommended for detection of nm23-H4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
