Application
Western blot analysis of FAIM2 expression in non-transfected: (A) and human FAIM2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of FAIM2 expression in human brain tissue extract in the absence (A) and the presence (B) of immunizing peptide, mouse brain (C) and rat brain (D) tissue extracts.| Product Name | FAIM2 Antibody (H-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of FAIM2 of mouse, rat and human origin by WB, IP, IF and IHC(P); also reactive with additional species, including canine |
| Modification Notes | FAIM2 (Fas apoptotic inhibitory molecule 2), also known as LFG (Protein lifeguard), TMBIM2 (Transmembrane BAX inhibitor motif-containing protein 2) and NMP35 (neural membrane protein 35), is a 316 amino acid multipass membrane protein that uniquely protects cells from Fas-induced apoptosis. Though widely expressed, FAIM2 expression is highest in hippocampus. FAIM2 contains seven transmembrane domains and resembles Bax Inhibitor-1, another anti-apoptotic protein. Overexpression of FAIM2 results in decreased caspase activation and reduced incidence of programmed cell death. Though mechanistically related to the Fas signal, FAIM2 does not protect cells from apoptosis that is mediated by TNF-å signaling. FAIM2 specifically regulates apoptosis by binding to the FAS receptor. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of FAIM2 of mouse rat and human origin by WB IP IF and IHC(P) also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of FAIM2 of mouse, rat and human origin by WB, IP, IF and IHC(P); also reactive with additional species, including canine |
| Entrez Gene | 23017 |
|---|
Application
Western blot analysis of FAIM2 expression in non-transfected: (A) and human FAIM2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of FAIM2 expression in human brain tissue extract in the absence (A) and the presence (B) of immunizing peptide, mouse brain (C) and rat brain (D) tissue extracts.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
