Application
Western blot analysis of Ovol1 expression in non-transfected: (A) and human Ovol1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Ovol1 expression in Jurkat whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining.| Product Name | Ovol1 Antibody (W-18) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The Ovo family of zinc-finger transcription factors encode evolutionarily conserved genes including those from Caenorhabditis elegans, Drosophila melanogaster, mouse and human. Members of the Ovo family include Ovol1 and Ovol2. Ovol1 acts as a transcriptional repressor by interacting with key developmental signaling pathways such as Wnt and TGF-∫/BMP. Specifically, Ovol1 represses c-Myc and Id2 genes and establishes a balance between proliferation and differentiation of progenitor cells. Deletion of Ovol1 in mice leads to germ cell degeneration and defective sperm production in adult males. Ovol1 has also been shown to repress itself as well as Ovol2, which is thought to regulate neural development and vascular angiogenesis during embryogenesis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Ovol1 of human, rat and canine origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against Ovol1 of human origin |
|---|---|
| Tissue Specificity | raised against Ovol1 of human origin |
| Entrez Gene | 5017 |
|---|
Application
Western blot analysis of Ovol1 expression in non-transfected: (A) and human Ovol1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Ovol1 expression in Jurkat whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining.| Application Notes | recommended for detection of Ovol1 of human, rat and canine origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
