Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human parathyroid gland tissue showing nuclear and cytoplasmic staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of SIAH-1 and SIAH-2 expression in HISM whole cell lysate.Application
Western blot analysis of human recombinant SIAH-1/2 fusion protein.| Product Name | SIAH-1/2 Antibody (FL-282) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | SIAH, the human homolog of the Drosophila seven in absentia (sina) gene, is a tumor suppressor protein that is expressed in intestinal epithelium and activated during apoptosis. Human SIAH proteins are produced as two distinct gene products, SIAH-1, and the slightly larger protein SIAH-2, which share a highly conserved C-terminal sequence and differ in their N-terminal regions. SIAH-1 contains an N-terminal RING-finger domain, which is required for proteolysis, and a cystein-rich C-terminal domain, which regulates oligomerization and SIAH binding to target proteins. As a tumor suppressor, SIAH-1 binds DCC (deleted in colorectal cancer) and regulates DCC degradation via the ubiquitin-proteasome pathway. SIAH-1 also binds a Bcl-2 related protein, Bag-1, thereby inhibiting cell growth. The majority of SIAH-1 is localized to the nucleus, however a small percentage is detected in the cytoplasm. This nuclear localization suggests that SIAH proteins may interact with other nuclear matrix proteins and DNA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of SIAH-1 and SIAH-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-282 representing full length SIAH-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-282 representing full length SIAH-1 of human origin |
| Entrez Gene | 6477 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human parathyroid gland tissue showing nuclear and cytoplasmic staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of SIAH-1 and SIAH-2 expression in HISM whole cell lysate.
Application
Western blot analysis of human recombinant SIAH-1/2 fusion protein.| Application Notes | recommended for detection of SIAH-1 and SIAH-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
