Application
Antibodies tested include p-Ribosomal Protein S6 (Ser 235): (A,B,C) and Ribosomal Protein S6 (C-8): (D,E,F).Application
Western blot analysis of phosphorylated Ribosomal Protein S6 expression in untreated (A) and serum-treated (B) 293 whole cell lysates.Application
Western blot analysis of Ribosomal Protein S6 phosphorylation in Hep G2 whole cell lysate.Application
Western blot analysis of Ribosomal Protein S6 phosphorylation in non-transfected: (A) and mouse Ribosomal Protein S6 transfected: (B) 293T whole cell lysates.| Product Name | p-Ribosomal Protein S6 Antibody (Ser 235) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 235 of Ribosomal Protein S6 of human origin |
| Modification | Phosphoralated |
| Modification Notes | The genes encoding for mammalian ribosomal proteins comprise multigene families that consist predominantly of multiple processed pseudogenes and one functional intron-containing gene within their coding regions. The RPS6 gene gives rise to Ribosomal Protein S6 (also designated RPS6). RPS6 is the major substrate of protein kinases in eukaryotic ribosomes. Sequence comparison has identified RPS6 as the equivalent of the Ribosomal Protein S10 from Saccharomyces cerevisiae. The sequence comparison of ribosomal proteins from evolutionarily distant eukaryotes, such as yeast and human, indicates that the structure and probably the function of RPS6 has been highly conserved. RPS6 phosphorylation is stimulated by growth factors, tumor promoting agents and mitogens. It is dephosphorylated at growth arrest. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| recommended for detection of Ser 235 phosphorylated Ribosomal Protein S6 of mouse, rat and human origin by WB and IP: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 235 of Ribosomal Protein S6 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 235 of Ribosomal Protein S6 of human origin |
| Entrez Gene | 6194 |
|---|
Application
Antibodies tested include p-Ribosomal Protein S6 (Ser 235): (A,B,C) and Ribosomal Protein S6 (C-8): (D,E,F).
Application
Western blot analysis of phosphorylated Ribosomal Protein S6 expression in untreated (A) and serum-treated (B) 293 whole cell lysates.
Application
Western blot analysis of Ribosomal Protein S6 phosphorylation in Hep G2 whole cell lysate.
Application
Western blot analysis of Ribosomal Protein S6 phosphorylation in non-transfected: (A) and mouse Ribosomal Protein S6 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Ser 235 phosphorylated Ribosomal Protein S6 of mouse, rat and human origin by WB and IP: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
