Application
Western blot analysis of SPAK expression in mouse liver tissue extract.Application
Western blot analysis of SPAK expression in non-transfected (A) and human SPAK transfected (B) 293 whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung carcinoma tissue showing cytoplasmic localization.| Product Name | SPAK Antibody (Y-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of SPAK of mouse and human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/threonine (Ser/Thr) protein kinases. SPAK, also known as STK39 (serine threonine kinase 39), DCHT or PASK, is a 547 amino acid protein that localizes to both the cytoplasm and the nucleus and contains one protein kinase domain. Expressed predominately in pancreas, brain, heart, lung, liver and testis, SPAK functions as a Ser/Thr protein kinase that catalyzes the ATP-dependent phosphorylation of target proteins and is thought to be involved in mediating stress-activated signals. The gene encoding SPAK maps to human chromosome 2, which houses over 1,400 genes and comprises nearly 8% of the human genome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of SPAK of mouse and human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of SPAK of mouse and human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 27347 |
|---|
Application
Western blot analysis of SPAK expression in mouse liver tissue extract.
Application
Western blot analysis of SPAK expression in non-transfected (A) and human SPAK transfected (B) 293 whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung carcinoma tissue showing cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
