Application
Western blot analysis of G3BP1 expression in Jurkat whole cell lysate.Application
Western blot analysis of G3BP1 expression in non-transfected 293T: (A), human G3BP1 transfected 293T: (B) and A-431 (C) whole cell lysates.Application
Western blot analysis of G3BP1 expression in 293T whole cell lysate.| Product Name | G3BP1 Antibody (H-94) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 158-251 mapping within an internal region of G3BP1 of human origin |
| Modification Notes | G3BP1 (GTPase activating protein (SH3 domain) binding protein 1), also known as G3BP or HDH-VIII, is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. One of several DNA-unwinding enzymes, G3BP1 functions as a sequence-specific, phosphorylation-dependent helicase that unwinds partial RNA and DNA duplexes containing hanging 3’- or 5’- ends. G3BP1 uses magnesium as a cofactor and, in addition to its helicase activity, acts as an endoribonuclease that cleaves mRNA between adenine and cytosine residues at the 3’-UTR. An element of the Ras signaling pathway, G3BP1 binds to the SH3 domain of Ras GTPase-activating protein (Ras GAP) in proliferating cells, thereby regulating Ras signaling events in developing tissues. Due to its involvement in both DNA replication and signaling pathways within the cell, G3BP1 expression is implicated in the pathogenesis of several cancers, including esophageal squamous carcinoma. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of G3BP1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 158-251 mapping within an internal region of G3BP1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 158-251 mapping within an internal region of G3BP1 of human origin |
| Entrez Gene | 10146 |
|---|
Application
Western blot analysis of G3BP1 expression in Jurkat whole cell lysate.
Application
Western blot analysis of G3BP1 expression in non-transfected 293T: (A), human G3BP1 transfected 293T: (B) and A-431 (C) whole cell lysates.
Application
Western blot analysis of G3BP1 expression in 293T whole cell lysate.| Application Notes | recommended for detection of G3BP1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
