Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human ovary tissue showing nuclear staining of ovarian stroma cells.Application
Western blot analysis of Histone H10 expression in L8 whole cell lysate.Application
Western blot analysis of Histone H10 expression in Sol8 whole cell lysate.| Product Name | Histone H10 Antibody (H-85) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-85 mapping at the N-terminus of Histone H10 of human origin |
| Modification Notes | Histone H1° (H1 histone family, member 0) is a lysine rich member of the H1 family of linker histones. The H1 family of proteins interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histone H1° is a unique variant considered a replacement linker histone which is expressed and incorporated into chromatin in the absence of DNA replication. In contrast, the majority of somatic H1 histones are replication dependent variants found in proliferating cells. Histone H1° is expressed in cells that are in the terminal stages of differentiation or that have low rates of cell division. Unlike other differentiation-specific linker histones which demonstrate tissue and species-specific expression, Histone H1° is widely expressed in many tissues in most vertebrates. Histone H1° is derived from an intronless gene, H1F0 in human which has been mapped to chromosome 22q13.1. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Histone H10 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-85 mapping at the N-terminus of Histone H10 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-85 mapping at the N-terminus of Histone H10 of human origin |
| Entrez Gene | 3005 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human ovary tissue showing nuclear staining of ovarian stroma cells.
Application
Western blot analysis of Histone H10 expression in L8 whole cell lysate.
Application
Western blot analysis of Histone H10 expression in Sol8 whole cell lysate.| Application Notes | recommended for detection of Histone H10 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
