Application
Western blot analysis of frizzled-1 expression in HeLa whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human prostate carcinoma tissue showing cytoplasmic and membrane localization.| Product Name | frizzled-1 Antibody (V-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of frizzled-1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | The frizzled gene, originally identified in Drosophila melanogaster, is involved in the development of tissue polarity. The mammalian homolog of frizzled as well as several secreted mammalian frizzled-related proteins (FRPs) have been described. The frizzled proteins contain seven transmembrane domains, a cysteine-rich domain in the extracellular region and a carboxy terminal Ser/Thr-xxx-Val motif. They function as receptors for Wnt and are generally coupled to G proteins. The frizzled-1 gene is expressed in adult heart, placenta, lung, kidney, pancreas, prostate and ovary and in fetal lung and kidney. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of frizzled-1 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of frizzled-1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 8321 |
|---|
Application
Western blot analysis of frizzled-1 expression in HeLa whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human prostate carcinoma tissue showing cytoplasmic and membrane localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
