Application
Western blot analysis of TCFL5 expression in Hep G2 whole cell lysate.| Product Name | TCFL5 Antibody (V-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of TCFL5 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | Transcription factors containing basic helix-loop-helix (bHLH) motifs are responsible for the regulation of neurogenesis, cardiogenesis, myogenesis, differentiation and cell proliferation. TCFL5 [transcription factor-like 5 (basic helix-loop-helix)], also known as cha transcription factor, HPV-16 E2-binding protein 1, CHA, Figlb, E2BP-1 or MGC46135, is a 500 amino acid protein belonging to the basic helix-loop-helix (bHLH) family. TCFL5 localizes to the nucleus and may function as a transcription factor during early spermatogenesis. TCFL5 is known to form a complex with upstream stimulatory factor (USF)-1, thereby negatively regulating USF-dependent transcription. Six isoforms of TCFL5 exist due to alternative splicing; isoform 2 is pancreas specific and isoform 3 is testis specific showing expression in primary spermatocytes at the pachytene stage. Antibodies against TCFL5 have been identified in patients with Chagas' disease, which is caused by Trypanosoma cruzi infection. The gene encoding TCFL5 maps to human chromosome 20q13.3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of TCFL5 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of TCFL5 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 10732 |
|---|
Application
Western blot analysis of TCFL5 expression in Hep G2 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
