Application
Western blot analysis of HMG-3 expression in human placenta tissue extract.| Product Name | HMG-3 Antibody (X-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of HMG-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Modification Notes | The HMGB family, whose members include HMG-1, HMG-2, HMG-3 and HMG-4, is a highly conserved group of chromatin-associated proteins. Evidence suggests that the binding of HMG proteins to DNA induces alterations in the DNA architecture including DNA bending and unwinding of the helix. HMG proteins synergize with Oct-2, members of the NF˚B family, ATF-2 and c-Jun to activate transcription. Other studies indicate that phosphorylation of HMG proteins is required to stimulate the transcriptional activity of HMG target proteins. HMG proteins bind single-stranded DNA, but are able to induce conformational changes in double-stranded DNA. HMG-3, also known as HMGB3 (High mobility group protein B3), is a 200 amino acid protein that localizes to the nucleus and is expressed in hematopoietic stem cells. As a member of a family of chromatin-binding proteins, HMG-3 facilitates transcription factor binding by altering DNA structure. HMG-3 may play a role in regulating proliferation and differentiation of certain cell lines. Like all other HMGB family proteins, HMG-3 contains two HMG box DNA-binding domains which can bind DNA either in a sequence-specific manner, or without sequence specificity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of HMG-3 of mouse rat and human origin by WB IP IF IHC(P) and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of HMG-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 3149 |
|---|
Application
Western blot analysis of HMG-3 expression in human placenta tissue extract.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
