Application
Western blot analysis of NARG1L expression in Hep G2 whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human muscle tissue showing nuclear and cytoplasmic localization.| Product Name | NARG1L Antibody (G-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of NARG1L of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | They cytoplasmic protein NARG1 (NMDA (N-methyl-d-aspartate) receptor-regulated gene 1) interacts with ARD1 or ARD2 to form a complex, which exhibits N-terminal (alpha) acetyltransferase activity. This complex interacts with ribosomal subunits functioning in cotranslational acetylation. During apoptosis, both NARG1 and ARD1 are cleaved by caspases, which results in decreased acetyltransferase activity. Knockdown of NARG1 in HeLa cells leads to apoptosis, indicating that properly functioning NARG1 is essential for cell viability. NARG1 is expressed at high levels in dividing tissues such as bone marrow, testis and embryonal brain and it is overexpressed in papillary thyroid carcinomas. The NARG1-like protein (NARG1L) is an 864 amino acid protein that contains 7 TPR repeats and may also be a component of a complex that displays N-terminal acetyltransferase activity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of NARG1L of mouse rat and human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of NARG1L of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 79612 |
|---|
Application
Western blot analysis of NARG1L expression in Hep G2 whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human muscle tissue showing nuclear and cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
