Application
Western blot analysis of Rictor expression in SK-BR-3 whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human testis tissue showing cytoplasmic localization.| Product Name | Rictor Antibody (V-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Rictor of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | FRAP is a large protein kinase that is the mammalian target of rapamycin, an immunosuppressant that blocks vessel restenosis and also has potential anticancer applications. Rapamycin-insensitive companion of FRAP, also designated Rictor, shares homology with pianissimo from D. discoidieum, STE20p from S. pombe, and AVO3p from S. cerevisiae. Rictor forms a complex with FRAP, which is important in cell growth regulation as it integrates growth factor and nutrient derived signals. The Rictor-FRAP complex plays a role in PKCå phosphorylation, directly phosphorylates Akt/PKB on Ser473 in vitro and facilitates Thr308 phosphorylation by PDK1. It also may function as a drug target in tumors that have lost expression of PTEN, a tumor suppressor that opposes activation of Akt/PKB. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Rictor of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Rictor of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 253260 |
|---|
Application
Western blot analysis of Rictor expression in SK-BR-3 whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human testis tissue showing cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
