Application
Western blot analysis of Eap45 expression in mouse embryo (A) and mouse ovary (B) tissue extracts.| Product Name | Eap45 Antibody (T-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Vacuolar sorting proteins (VPSs) are required for trafficking normal endocytic and biosynthetic proteins to the vacuole and also play an important role in the budding process of cells. The ESCRT-II (endosomal sorting complex required for transport II) complex, which is involved in endocytosis of ubiquinated membrane proteins, is formed by Eap45, EAP30, and VPS25. These vacuolar sorting proteins are also involved in a multiprotein complex with RNA ploymerase II elongation factor (ELL). Eap45 (ELL-associated protein of 45 kDa), also known as vacuolar protein-sorting-associated protein 36, is a 386 amino acid protein containing a GLUE (GRAM-like ubiquitin-binding in Eap45) domain that mediates the binding to ubiquitin and phophoinosides. Since Eap45 colocalizes with ubiquinated proteins on late endosomes, it is likely that Eap45 plays a role in the endosomal sorting of ubiquniated cargo. There are two isoforms of Eap45 that exist as a result of alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Eap45 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of Eap45 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of Eap45 of human origin |
| Entrez Gene | 51028 |
|---|
Application
Western blot analysis of Eap45 expression in mouse embryo (A) and mouse ovary (B) tissue extracts.| Application Notes | recommended for detection of Eap45 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
