Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human upper stomach tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of COL6A2 expression in non-transfected: (A) and human COL6A2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of COL6A2 expression in non-transfected: (A) and mouse COL6A2 transfected: (B) 293T whole cell lysates.| Product Name | COL6A2 Antibody (D-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within an internal region of Collagen α2 Type VI of human origin |
| Modification Notes | Collagens (COLs) are fibrous, extracellular matrix proteins with high tensile strength that function as the major components of connective tissue, such as tendons and cartilage. All COL proteins contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. There are several types of COL proteins, including fibril-forming interstitial COLs (types I, II, III and V), basement membrane COLs (type IV) and beaded filament COLs (type VI). COL6A2 (collagen, type VI, alpha 2), also known as PP3610, is a 1,019 amino acid secreted protein that contains three VWFA domains and functions as the second of three alpha chains that comprise the type VI COL protein complex. Existing as a trimer with two other type VI alpha proteins, COL6A2 acts as a cell-binding protein that plays an important role in the organization of matrix components. Defects in the gene encoding COL6A2 are associated with Bethlem myopathy (BM) and Ullrich congenital muscular dystrophy (UCMD). Multiple isoforms of COL6A2 exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Collagen α2 Type VI of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of Collagen &alpha 2 Type VI of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of Collagen α2 Type VI of human origin |
| Entrez Gene | 1292 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human upper stomach tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of COL6A2 expression in non-transfected: (A) and human COL6A2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of COL6A2 expression in non-transfected: (A) and mouse COL6A2 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Collagen α2 Type VI of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
