Application
Western blot analysis of Crk II phosphorylation in non-transfected: (A) and human Crk II transfected: (B) 293 whole cell lysates.Application
Western blot analysis of Crk II phosphorylation in non-transfected: (A) and mouse Crk II transfected: (B) 293T whole cell lysates.| Product Name | p-Crk II Antibody (Ser 41) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Ser 41 phosphorylated Crk II of mouse, rat and human origin by WB, IP and ELISA |
| Modification | Phosphoralated |
| Modification Notes | The v-Crk oncogene product shares homologous amino acid sequences, designated SH2 (for Src homology region 2) and SH3, with many molecules involved in signal transduction. The v-Crk cellular homolog, c-Crk, is a member of a newly emerging class of genes, including Nck and GRB2/ASH, which encode proteins that consist primarily of SH2 and SH3 domains. Two distinct human c-Crk cDNAs, designated Crk I and Crk II, have been identified and shown to represent alternative splice products of c-Crk. The major c-Crk transforming activity appears associated with c-Crk I p28 expression. IGF-I receptor kinase can induce phosphorylation of Crk II at Tyr 221. Human Crk II may also be phosphorylated at Ser 41. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 41 phosphorylated Crk II of mouse rat and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Ser 41 phosphorylated Crk II of mouse, rat and human origin by WB, IP and ELISA |
| Entrez Gene | 1398 |
|---|
Application
Western blot analysis of Crk II phosphorylation in non-transfected: (A) and human Crk II transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of Crk II phosphorylation in non-transfected: (A) and mouse Crk II transfected: (B) 293T whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
