| Product Name | TGase5 Antibody (H-15) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of TGase5 of human origin by WB, IF and IHC(P) |
| Modification Notes | Terminally differentiating mammalian epidermal cells acquire an insoluble, 10 to 20 nm thick protein deposit on the intracellular surface of the plasma membrane known as the cross-linked cell envelope (CE). The CE is a component of the epidermis that is generated through formation of disulfide bonds and gamma-glutamyl-lysine isodipeptide bonds, which are formed by the action of transglutaminases (TGases). TGases are intercellular localized, Ca2+-dependent enzymes, which catalyze the formation of isopeptide bonds by transferring an amine to a glutaminyl residue, thereby cross-linking glutamine residues and lysine residues in substrate proteins. TGases influence numerous biological processes, including blood coagulation, epidermal differentiation, seminal fluid coagulation, fertilization, cell differentiation and apoptosis. TGase5 (transglutaminase 5), also known as TGM5 or TGX, is a 720 amino acid cytoplasmic protein that uses calcium to catalyze the cross-linking of proteins and plays an important role in the formation of the cornified cell envelope of keratinocytes. Defects in the gene encoding TGase5 are associated with peeling skin syndrome acral type (APSS), an autosomal recessvie disease characterized by the continuous shedding of the outer layers of the epidermis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIFIHC |
| WB:1:500~1:2000 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of TGase5 of human origin by WB IF and IHC(P) |
|---|---|
| Tissue Specificity | recommended for detection of TGase5 of human origin by WB, IF and IHC(P) |
| Entrez Gene | 9333 |
|---|
| Application Notes | WB:1:500~1:2000 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
