Application
Western blot analysis of Glyoxalase II expression in non-transfected: (A) and human Glyoxalase II transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Glyoxalase II expression in non-transfected: (A) and mouse Glyoxalase II transfected: (B) 293T whole cell lysates.| Product Name | Glyoxalase II Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 61-110 mapping within an internal region of Glyoxalase II of human origin |
| Modification Notes | The glyoxal pathway plays a role in the detoxification of glucose degradation products (GDP). Glyoxalase I and Gyloxalase II (also designated hydroxyacyl glutathione hydrolase or HAGH) are members of the Gyloxalase family. The Gyloxalase II enzyme is a thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form reduced glutathione and D-lactic acid. It exists only as a monomer and binds two zinc ions per subunit. Glyoxalase II contains 260 amino acids. It is detected in the mitochondria and cytosol of mammals. Both Glyoxalase I and Gyloxalase II are detected at a higher activity level in breast cancer tissues than with matched unaffected tissues. This suggests that glyoxalase inhibitor drugs may be effective in the treatment of cancer. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Glyoxalase II of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 61-110 mapping within an internal region of Glyoxalase II of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 61-110 mapping within an internal region of Glyoxalase II of human origin |
| Entrez Gene | 3029 |
|---|
Application
Western blot analysis of Glyoxalase II expression in non-transfected: (A) and human Glyoxalase II transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Glyoxalase II expression in non-transfected: (A) and mouse Glyoxalase II transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Glyoxalase II of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
