Application
Western blot analysis of RIPX expression in non-transfected: (A) and mouse RIPX transfected: (B) 293T whole cell lysates.Application
Western blot analysis of RIPX expression in Jurkat whole cell lysate.| Product Name | RIPX Antibody (P-16) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against RIPX of human origin |
| Modification Notes | RIPX (Rap 2-interacting protein x), also known as RUFY3 (RUN and FYVE domain containing 3) or Singar1, is a 469 amino acid protein that contains one RUN (RPIP8, unc-14 and NESCA) domain and is highly expressed in brain tissue. Localized to both the cell projection and to the lamellipodia and filopodia of growth cones, RIPX is thought to play a role neuronal development, specifically by mediating the formation of single axons, a process that maintains optimal neuronal polarity. RIPX interacts with PI 3-kinase p110å and PI 3-kinase p85å and, via this interaction, may be able to inhibit the formation of additional axons during neuronal maturation. Two isoforms of RIPX (one of which is partially phosphorylated) exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| recommended for detection of RIPX of mouse, rat, human and canine origin by WB, IP and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against RIPX of human origin |
|---|---|
| Tissue Specificity | raised against RIPX of human origin |
| Entrez Gene | 22902 |
|---|
Application
Western blot analysis of RIPX expression in non-transfected: (A) and mouse RIPX transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of RIPX expression in Jurkat whole cell lysate.| Application Notes | recommended for detection of RIPX of mouse, rat, human and canine origin by WB, IP and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
