Application
Antibodies tested include p-Adducin α (Ser 726): (A, B, C) and Adducin α (4D1): (D, E, F).Application
Western blot analysis of Adducin α phosphorylation in non-transfected: (A) and mouse Adducin α transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung carcinoma tissue showing cytoplasmic staining.Application
Western blot analysis of phosphorylated Adducin α expression in untreated (A) and Doxorubicin-treated (B) HT-29 whole cell lysates.| Product Name | p-Adducin α Antibody (Ser 726) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 726 of Adducin α of human origin |
| Modification | Phosphoralated |
| Modification Notes | Adducins are a family of cytoskeleton proteins encoded by three genes (a, b and g). Adducin is a protein associated with the inner leaflet of the plasma membrane and is one of the proteins localized at the spectrin-actin junction of the membrane skeleton. Adducins promote association of spectrin with actin and cap the fast growing end of actin filaments. Adducins contain an N-terminal core, neck and C-terminal tail domains, are substrates for protein kinase A (PKA) and C (PKC), and bind to Ca2+/calmodulin. The major phosphorylation sites common to the Adducins are Ser 726 and Ser 713 in the C-terminal MARCKS-related domains of Adducin å and Adducin ∫, and they are phosphorylated by PKA and PKC, respectively. In addition, PKA phosphorylates Adducin a at Ser 408, 436 and 481. Calmodulin-binding is inhibited by phosphorylation of Adducin b that, in turn, inhibits the rate of phospho-rylation of Adducin ∫, but not Adducin å. Rho-kinase can phosphorylate Adducin å at Thr 445 and Thr 480 downstream of Rho in vivo. The phosphorylation of Adducin by Rho-kinase plays an important role in the regulation of membrane ruffling and cell motility. In addition, phosphorylation at Ser 726 of Adducin å is required for cleavage by caspase-3 . |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Ser 726 phosphorylated Adducin α of human origin and correspondingly phosphorylated Ser 724 of mouse and rat origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 726 of Adducin &alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 726 of Adducin α of human origin |
| Entrez Gene | 118 |
|---|
Application
Antibodies tested include p-Adducin α (Ser 726): (A, B, C) and Adducin α (4D1): (D, E, F).
Application
Western blot analysis of Adducin α phosphorylation in non-transfected: (A) and mouse Adducin α transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung carcinoma tissue showing cytoplasmic staining.
Application
Western blot analysis of phosphorylated Adducin α expression in untreated (A) and Doxorubicin-treated (B) HT-29 whole cell lysates.| Application Notes | recommended for detection of Ser 726 phosphorylated Adducin α of human origin and correspondingly phosphorylated Ser 724 of mouse and rat origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
