Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of Ig J Chain expression in non-transfected: (A) and mouse Ig J Chain transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Ig J chain expression in mouse spleen tissue extra ct.Application
Western blot analysis of Ig J chain expression in mouse spleen (A) and mouse liver (B) tissue extracts.| Product Name | Ig J chain Antibody (M-159) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-159 representing full length Ig J chain of mouse origin |
| Modification Notes | The regions of relatively constant sequence beyond the variable regions of Immunoglobulin are termed constant regions (C regions) and are present in both the heavy and light chains. With few exceptions, the sites of attachment for carbohydrates to immunoglobulin are located in the constant region. The constant regions also serve to hold the variable regions on both heavy and light chain together by virtue of the disulfide bond between them. The immunoglobulin J chain (Ig J chain) is a linker protein for two monomer units of either immunoglobulin alpha (IgA) or mu (IgM) polypeptides. For IgA the J chained-joined dimer induces larger polymers whereas for the IgM pentamer it functions as a nucleating unit. The Ig J-chain is also important in binding these immunoglobulins to secretory components. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Ig J chain of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: WB:1:100~1:200 IHC:1:10~1:50 IF:1:20~1:100 ELISA:1:1000~5000 IP:1:20~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-159 representing full length Ig J chain of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-159 representing full length Ig J chain of mouse origin |
| Entrez Gene | 16069 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of Ig J Chain expression in non-transfected: (A) and mouse Ig J Chain transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Ig J chain expression in mouse spleen tissue extra ct.
Application
Western blot analysis of Ig J chain expression in mouse spleen (A) and mouse liver (B) tissue extracts.| Application Notes | recommended for detection of Ig J chain of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: WB:1:100~1:200 IHC:1:10~1:50 IF:1:20~1:100 ELISA:1:1000~5000 IP:1:20~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
