Application
Western blot analysis of APPL2 expression in Hs 181.Tes whole cell lysate (A) and truncated human recominant APPL2 fusion protein (B).Application
Western blot analysis of APPL2 expression in non-transfected: (A) and mouse APPL2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of APPL2 expression in non-transfected: (A) and human APPL2 transfected: (B) 293T whole cell lysates.| Product Name | APPL2 Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 605-664 mapping at the C-terminus of APPL2 of human origin |
| Modification Notes | The APPL family of proteins are involved in linking, trafficking and signaling downstream of tyrosine kinase receptors. APPL1, also designated adaptor protein containing pH domain, PTB domain and leucine zipper motif 1; APPL; or DCC interacting protein 13å (DIP13å), and APPL2, also designated adaptor protein containing pH domain, PTB domain and leucine zipper motif 2 or DCC interacting protein 13∫ (DIP13∫), are involved in the coupling of epidermal growth factor (EGF) signaling and chromatin remodeling in the nucleus. They associate with GTPase Rab 5 and are released from the plasma membrane and translocated to the nucleus. In the nucleus, APPL1 and APPL2 associate with NuRD/MeCP1 and are essential for cell growth and proliferation. APPL2 also associates with follicle stimulating hormone receptor (FSHR). APPL2 is highly expressed in heart, brain, skeletal muscle, and kidney. APPL2 shares 54% homology with APPL1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of APPL2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 605-664 mapping at the C-terminus of APPL2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 605-664 mapping at the C-terminus of APPL2 of human origin |
| Entrez Gene | 55198 |
|---|
Application
Western blot analysis of APPL2 expression in Hs 181.Tes whole cell lysate (A) and truncated human recominant APPL2 fusion protein (B).
Application
Western blot analysis of APPL2 expression in non-transfected: (A) and mouse APPL2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of APPL2 expression in non-transfected: (A) and human APPL2 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of APPL2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
