Application
Western blot analysis of DNASE1L3 expression in mouse liver tissue extract.| Product Name | DNASE1L3 Antibody (H-77) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 21-97 mapping near the N-terminus of DNASE1L3 of human origin |
| Modification Notes | DNASE1L3 (deoxyribonuclease I-like 3), also known as LSD (liver and spleen DNase), DHP2, DNase ©, DNase Y or DNAS1L3, is a member of the DNase I family of Ca2+/Mg2+-dependent endonucleases. DNASE1L3 localizes to the nucleus and is expressed in liver, spleen, thymus, small intestine, kidney, bone marrow and lymph node. DNASE1L3 cleaves nuclear chromatin internucleosomally and is believed to play a role in DNA breakdown during apoptosis. DNASE1L3 cleaves single and double-stranded DNA, producing 3’-OH/5’-P ends. The endonuclease activity of DNASE1L3 can be enhanced by association with å-actinin-4 and repressed by Poly-ADP-ribosylation by PARP-1. PARP-1 activity can be inactivated in the execution phase of apoptosis by caspase-like proteases, thereby relieving the inhibition of DNASE1L3. DNASE1L3 may also be inhibited by zinc but, in contrast with DNase 1, it is not inhibited by monomeric actin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of DNASE1L3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and equine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 21-97 mapping near the N-terminus of DNASE1L3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 21-97 mapping near the N-terminus of DNASE1L3 of human origin |
| Entrez Gene | 1776 |
|---|
Application
Western blot analysis of DNASE1L3 expression in mouse liver tissue extract.| Application Notes | recommended for detection of DNASE1L3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and equine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
