Application
Western blot analysis of Sec15A expression in non-transfected: (A) and human Sec15A transfected: (B) 293T whole cell lysates.| Product Name | Sec15A Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 221-280 mapping within an internal region of Sec15A of human origin |
| Modification Notes | Exocytosis, crucial in membrane trafficking, mediates hormone and neurotransmitter secretion out of the cell as well as the incorporation of membrane proteins and lipids to the plasma membrane. It is crucial for cell-cell communication, cell growth and cell polarity. The exocyst complex is a multi-protein complex that consists of Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70 and Exo84 and is essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. The exocyst complex inhibits tubulin polymerization in vitro which implicates the exocyst in modulating microtubule dynamics underlying exocytosis. Sec15A (also known as Sec15L or Sec15p) and Sec15B (also designated Sec15L2) both belong to the SEC15 family of proteins. Sec15 can colocalize with Rab11 (a recycling endosome marker) and exhibits a GTP-dependent interaction with the Rab11 GTPase. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Sec15A of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 221-280 mapping within an internal region of Sec15A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 221-280 mapping within an internal region of Sec15A of human origin |
| Entrez Gene | 54536 |
|---|
Application
Western blot analysis of Sec15A expression in non-transfected: (A) and human Sec15A transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Sec15A of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
