Application
Western blot analysis of SEMA7A expression in human erythocyte whole cell lysate.| Product Name | SEMA7A Antibody (H-41) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 371-411 mapping within an internal region of SEMA7A of human origin |
| Modification Notes | Semaphorins comprise a family of cell surface and secreted proteins that are conserved from insects to humans (1,2). Members of this family of proteins are approximately 750 amino acids in length (including signal sequences) and are defined by a conserved extracellular “semaphorin” domain of approximately 500 amino acids containing 14-16 cysteines, many blocks of conserved sequences and no obvious repeats (2-4). The transmembrane semaphorins are characterized by an additional 80 amino acid transmembrane domain and an 80-110 amino acid cytoplasmic domain (4). These semaphorin proteins regulate the growth of the axons during embryogenesis by repelling growth cones from regions of high semaphorin expression (2,4). Also designated CD108, Semaphorin 7A (SEMA7A) promotes axonal growth in the central nervous system and plays a critical role in negative regulation of T cell activation and function. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Semaphorin-7A of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 371-411 mapping within an internal region of SEMA7A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 371-411 mapping within an internal region of SEMA7A of human origin |
| Entrez Gene | 8482 |
|---|
Application
Western blot analysis of SEMA7A expression in human erythocyte whole cell lysate.| Application Notes | recommended for detection of Semaphorin-7A of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
