Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lower stomach tissue showing nuclear and cytoplasmic staining of glandular cells.Application
Western blot analysis of ERRγ expression in non-transfected: (A) and human ERRγ transfected: (B) 293T whole cell lysates.| Product Name | ERRγ Antibody (H-38) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 81-118 mapping near the N-terminus of ERRγ of human origin |
| Modification Notes | Estrogen and progesterone receptors are members of a family of transcription factors that are regulated by the binding of their cognate ligands. The interaction of hormone-bound estrogen receptors with estrogen responsive elements (EREs) alters transcription of ERE-containing genes. Estrogen receptor-related proteins (ERRå, ∫ and ©) are orphan nuclear receptors. Like estrogen receptors, ERRs bind specifically to EREs to activate reporter genes. EREs are constitutively active without binding to estrogen. The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (PR-A and PR-B), which arise from alternative splicing. In most cells, PR-B functions as a transcriptional activator of progesterone-responsive genes, whereas PR-A functions as a transcriptional inhibitor of all steroid hormone receptors. mPR is a membrane progestin receptor. The predicted 436-amino acid ERR© protein which presumably localizes to the nucleus, is expressed in the heart, kidney, brain, lung, bone marrow, adrenal gland, trachea, spinal cord and thyroid gland tissues. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of ERRγ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 81-118 mapping near the N-terminus of ERR&gamma of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 81-118 mapping near the N-terminus of ERRγ of human origin |
| Entrez Gene | 2104 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lower stomach tissue showing nuclear and cytoplasmic staining of glandular cells.
Application
Western blot analysis of ERRγ expression in non-transfected: (A) and human ERRγ transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of ERRγ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
