Application
Western blot analysis of ATP5A expression in HeLa whole cell lysate.| Product Name | ATP5A Antibody (H-252) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 302-553 mapping at the C-terminus of ATP5A of human origin |
| Modification Notes | Mitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds. ATPases contain two linked complexes: F1, the hydrophilic catalytic core; and F0, the membrane-embedded protein channel. F1 consists of three å chains and three ∫ chains, which are weakly homologous, as well as one © chain, one ∂ chain and one e chain. F0 consists of three subunits: a, b and c. The å chain of F1 is a regulatory subunit that contains 509 amino acids. Mitochondrial ATPase å chain (ATP5A) localizes to the mitochondria and catalyzes ATP synthesis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ATP5A of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 302-553 mapping at the C-terminus of ATP5A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 302-553 mapping at the C-terminus of ATP5A of human origin |
| Entrez Gene | 498 |
|---|
Application
Western blot analysis of ATP5A expression in HeLa whole cell lysate.| Application Notes | recommended for detection of ATP5A of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
