Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing membrane localization.Application
Western blot analysis of TMED5 expression in non-transfected 293T: (A), human TMED5 transfected 293T: (B), Hep G2 (C) and HeLa (D) whole cell lysates.| Product Name | TMED5 Antibody (N-13) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the N-terminus of TMED5 of human origin |
| Modification Notes | TMED5 (transmembrane emp24 domain-containing protein 5) is a 229 amino acid single-pass type I membrane protein that belongs to the EMP24/GP25L family and contains one GOLD domain. The gene that encodes TMED5 contains nearly 31,000 bases and maps to human chromosome 1p22.1. As the largest human chromosome, chromosome 1 spans about 260 million base pairs and makes up approximately 8% of the human genome. There are about 3,000 genes on chromosome 1, and considering the great number of genes there are also a large number of diseases associated with chromosome 1. Notably, the rare aging disease Hutchinson-Gilford progeria is associated with the LMNA gene which encodes lamin A. When defective, the LMNA gene product can build up in the nucleus and cause characteristic nuclear blebs. The MUTYH gene is located on chromosome 1 and is partially responsible for familial adenomatous polyposis. Stickler syndrome, Parkinsons, Gaucher disease and Usher syndrome are also associated with chromosome 1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of TMED5 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with other TMED family members also reactive with additional species, including equine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the N-terminus of TMED5 of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the N-terminus of TMED5 of human origin |
| Entrez Gene | 50999 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing membrane localization.
Application
Western blot analysis of TMED5 expression in non-transfected 293T: (A), human TMED5 transfected 293T: (B), Hep G2 (C) and HeLa (D) whole cell lysates.| Application Notes | recommended for detection of TMED5 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with other TMED family members also reactive with additional species, including equine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
