Application
Western blot analysis of ALAS-E expression in human heart (A), rat heart (B) and rat liver (C) tissue extracts.Application
Western blot analysis of ALAS-E expression in non-transfected: (A) and mouse ALAS-E transfected: (B) 293T whole cell lysates and mouse heart tissue extract (C).| Product Name | ALAS-E Antibody (M-100) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 61-160 mapping near the N-terminus of ALAS-E of mouse origin |
| Modification Notes | 5-aminolevulinate synthase 1 (ALAS-H) and 2 (ALAS-E) are two isoforms of ALAS, an enzyme catalyzing the first step of the heme biosynthetic pathway in mammals. The erythroid-specific isoenzyme, ALAS-E, regulates the first step of hematopoietic cell differentation and iron metabolism in the liver. ALAS-H is a housekeeping protein which mediates synthesis of early heme in the mitochondria of most cells. Succinyl CoA associates with ALAS-E in protein conformation change and translocation of ALAS-E into the mitochondria and does not interact with ALAS-H. The ALAS-E 5'-flanking region contains binding sites for nuclear activators such as GATA-1, NF-E2 and EKLF. Since the ALAS gene maps to the X chromosome, mutation of the gene leads to the pyridoxine-refractory X-linked sideroblastic anemia. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of precursor and mature ALAS-E of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 61-160 mapping near the N-terminus of ALAS-E of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 61-160 mapping near the N-terminus of ALAS-E of mouse origin |
| Entrez Gene | 212 |
|---|
Application
Western blot analysis of ALAS-E expression in human heart (A), rat heart (B) and rat liver (C) tissue extracts.
Application
Western blot analysis of ALAS-E expression in non-transfected: (A) and mouse ALAS-E transfected: (B) 293T whole cell lysates and mouse heart tissue extract (C).| Application Notes | recommended for detection of precursor and mature ALAS-E of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
