Application
Western blot analysis of Flightless I expression in SJRH30 whole cell lysate.Application
Western blot analysis of Flightless I expression in non-transfected: (A) and mouse Flightless I transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Flightless I expression in Sol8 (A) and L8 (B) whole cell lysates.| Product Name | Flightless I Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of Flightless I of human origin |
| Modification Notes | The Drosophila melanogaster Flightless I gene is required for normal cellularization of the syncytial blastoderm in early embryogenesis and in the structural organization of indirect flight muscle. The Flightless I protein contains an Actin-binding domain with homology to the gelsolin family and is likely to be involved in Actin cytoskeletal rearrangements. Flightless I also contains an N-terminal leucine-rich repeat protein-protein interaction domain. The Flightless I protein localizes predominantly to the nucleus and translocates to the cytoplasm following serum stimulation. In cells stimulated to migrate, the Flightless I protein co-localizes with b-tubulin- and Actin-based structures. The human FLI gene is mapped within the Smith-Magenis microdeletion region of chromosome 17 at 17p11.2. Smith-Magenis syndrome is characterized by short stature, brachydactyly, developmental delay, dysmorphic features, sleep disturbances and behavioral problems. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Flightless I of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of Flightless I of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of Flightless I of human origin |
| Entrez Gene | 2314 |
|---|
Application
Western blot analysis of Flightless I expression in SJRH30 whole cell lysate.
Application
Western blot analysis of Flightless I expression in non-transfected: (A) and mouse Flightless I transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Flightless I expression in Sol8 (A) and L8 (B) whole cell lysates.| Application Notes | recommended for detection of Flightless I of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
