Application
Western blot analysis of NDRG2 expression in non-transfected: (A) and mouse NDRG2 transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).Application
Western blot analysis of NDRG2 expression in non-transfected 293: (A), human NDRG2 transfected 293: (B) and Jurkat (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human epididymis tissue showing cytoplasmic and nuclear staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of NDRG2 expression in non-transfected: (A) and human NDRG2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of NDRG2 expression in mouse brain tissue extract.Application
Western blot analysis of NDRG2 expression in non-transfected: (A) and human NDRG2 transfected: (B) 293 whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing perinuclear localization.| Product Name | NDRG2 Antibody (H-40) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 104-143 mapping within an internal region of NDRG2 of human origin |
| Modification Notes | The N-myc downstream regulated gene (NDRG) family is comprised of four members, namely NDRG1, NDRG2, NDRG3 and NDRG4, all of which share 57-65% homology. NDRG2 (NDRG family member 2), also known as SYLD, is a 371 amino acid protein that localizes to both the cytoplasm and the perinuclear region in neurons. Expressed at high levels in heart, brain, dendritic cells, salivary gland and skeletal muscle and at lower levels in liver and kidney, NDRG2 is thought to be involved in dendritic and neuronal cell differentiation and outgrowth. Additionally, NDRG2 expression is downregulated in a variety of carcinomas, including liver cancer, pancreatic cancer and meningioma, suggesting a possible role for NDRG2 in tumor suppression. NDRG2 is found in brain lesions of Alzheimer Disease (AD)-affected patients and is thought to be associated with the progression of AD. Five isoforms of NDRG2 exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of NDRG2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 104-143 mapping within an internal region of NDRG2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 104-143 mapping within an internal region of NDRG2 of human origin |
| Entrez Gene | 57447 |
|---|
Application
Western blot analysis of NDRG2 expression in non-transfected: (A) and mouse NDRG2 transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).
Application
Western blot analysis of NDRG2 expression in non-transfected 293: (A), human NDRG2 transfected 293: (B) and Jurkat (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human epididymis tissue showing cytoplasmic and nuclear staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of NDRG2 expression in non-transfected: (A) and human NDRG2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of NDRG2 expression in mouse brain tissue extract.
Application
Western blot analysis of NDRG2 expression in non-transfected: (A) and human NDRG2 transfected: (B) 293 whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing perinuclear localization.| Application Notes | recommended for detection of NDRG2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
