Application
Western blot analysis of RFX1 expression in HeLa whole cell lysate.Application
Immuno?fluorescence staining of methanol-fixed HeLa cells showing nuclear local?ization.Application
Western blot analysis of RFX1 expression in K-562 (A) and HeLa (B) nuclear extracts.| Product Name | RFX1 Antibody (H-230) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 61-290 mapping near the N-terminus of RFX1 of human origin |
| Modification Notes | EP is a regulatory enhancer element found in several promoters on viral genes, and similar sites are also present in cellular genes, including the MIF-1 binding site (MIE) of the human c-myc gene, the X box of MHC class II promoters and a binding site in the proliferating cell nuclear antigen promoter. The EP sites present in the X box of MHC class II promoters are distinctly nonpalindromic sequences that contain only a single EP-homologous half-site. The EP element is bound by an ubiquitous nuclear protein complex that consists of homo- and heterodimers involving the RFX1, RFX2 and RFX3 proteins. The RFX proteins represent an essential class II transcription factor family that shares several conserved regions, including the centrally located DNA-binding domain (DBD) and the D region found in the C-terminal part of these proteins which facilitates dimerization. RFX complexes can activate the enhancer elements of several HBV genes and also promote the induction of MHC class II genes in response to interferon-g stimulation. Two additional subunits, RFX5, RFX-B/Ank, are also involved in the RFX complexes, yet they bind additional elements in the X1 half of the X box. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of RFX1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 61-290 mapping near the N-terminus of RFX1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 61-290 mapping near the N-terminus of RFX1 of human origin |
| Entrez Gene | 5989 |
|---|
Application
Western blot analysis of RFX1 expression in HeLa whole cell lysate.
Application
Immuno?fluorescence staining of methanol-fixed HeLa cells showing nuclear local?ization.
Application
Western blot analysis of RFX1 expression in K-562 (A) and HeLa (B) nuclear extracts.| Application Notes | recommended for detection of RFX1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
