Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing cytoplasmic staining of Purkinje cells, cells in granular layer and cells in molecular layer.Application
Western blot analysis of BRD4 expression in HeLa whole cell lysate.| Product Name | BRD4 Antibody (H-250) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1031-1280 mapping near the C-terminus of Bromodomain-containing protein 4 of human origin |
| Modification Notes | Brd4 belongs to the BET family, a group of structurally related proteins containing two bromodomains. Through these two domains, Brd4 associates with mitotic chromosomes and its expression correlates with cell growth. Expression of Brd4 inhibits cell cycle progression from G(1) to S, due to binding to the largest subunit of replication factor C (RFC) to prevent DNA elongation. Altered Brd4 function correlates with poorly differentiated carcinoma, with aggresive phenotype and a highly lethal outcome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of BRD4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1031-1280 mapping near the C-terminus of Bromodomain-containing protein 4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1031-1280 mapping near the C-terminus of Bromodomain-containing protein 4 of human origin |
| Entrez Gene | 23476 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing cytoplasmic staining of Purkinje cells, cells in granular layer and cells in molecular layer.
Application
Western blot analysis of BRD4 expression in HeLa whole cell lysate.| Application Notes | recommended for detection of BRD4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
