Application
Western blot analysis of MAFbx expression in non-transfected: (A) and mouse MAFbx transfected: (B) 293T whole cell lysates.| Product Name | MAFbx Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of MAFbx of human origin |
| Modification Notes | Muscle atrophy F-box (MAFbx) is an E3 ubiquitin ligase that initiates ATP-dependent ubiquitin-mediated proteolysis and promotes muscle atrophy. MAFbx transcript is abundant in cardiac and skeletal muscle undergoing atrophy. MAFbx -/- mice are resistant to muscle atrophy. MAFbx is thought to recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation during skeletal muscle atrophy. It interacts with MyoD by ubiquitination via a sequence found in transcriptional coactivators and therefore may play an important role in the course of muscle differentiation by determining the abundance of MyoD. MAFbx is specifically expressed in cardiac and skeletal muscle. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MAFbx isoforms 1-3 and F-box only protein 25 isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of MAFbx of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of MAFbx of human origin |
| Entrez Gene | 114907 |
|---|
Application
Western blot analysis of MAFbx expression in non-transfected: (A) and mouse MAFbx transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of MAFbx isoforms 1-3 and F-box only protein 25 isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
