Application
Western blot analysis of CXXC5 expression in non-transfected: (A) and human CXXC5 transfected: (B) 293 whole cell lysates and HeLa (C), MCF7 (D) and K-562 (E) nuclear extracts.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing nuclear staining of neuronal and glial cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing perinuclear localization.Application
Western blot analysis of CXXC5 expression in non-transfected 293: (A), human CXXC5 transfected 293: (B) and HeLa (C) whole cell lysates.| Product Name | CXXC5 Antibody (S-12) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within an internal region of CXXC5 of human origin |
| Modification Notes | Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. CXXC5 (CXXC finger 5), also known as RINF or HSPC195, is a 322 amino acid protein that localizes to both the nucleus and the cytoplasm and contains one CXXC-type zinc finger. Interacting with Dvl-1, CXXC5 acts as a mediator of Wnt signaling in neural stem cells and is thought to participate in the activation of MAP kinase pathways. The gene encoding CXXC5 maps to human chromosome 5 and is expressed as multiple alternatively spliced isoforms. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of CXXC5 isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of CXXC5 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of CXXC5 of human origin |
| Entrez Gene | 51523 |
|---|
Application
Western blot analysis of CXXC5 expression in non-transfected: (A) and human CXXC5 transfected: (B) 293 whole cell lysates and HeLa (C), MCF7 (D) and K-562 (E) nuclear extracts.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing nuclear staining of neuronal and glial cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing perinuclear localization.
Application
Western blot analysis of CXXC5 expression in non-transfected 293: (A), human CXXC5 transfected 293: (B) and HeLa (C) whole cell lysates.| Application Notes | recommended for detection of CXXC5 isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
