Application
Western blot analysis of PUMAα/β expression in etposide treated HeLa (A) and PMA treated A-431 (B) whole cell lysates.Application
Immunofluorescence staining of normal mouse lymph node frozen section showing cytoplasmic and perinuclear staining.| Product Name | PUMAα/β Antibody (H-136) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 57-193 mapping at the C-terminus of PUMAα of human origin |
| Modification Notes | PUMA (Bcl-2 binding component 3, JFY1, PUMA/JFY1) is a mitochondrial pro-apoptotic Bcl-2 homology domain (BH3)-only protein that induces rapid apoptosis through a Bax- and mitochondria-dependent pathway. The PUMA gene encodes four proteins originating from different splice variants of the same transcript: PUMAå, ∫, © and ∂. Both PUMAå and PUMA∫ contain a BH3 domain, while PUMA© and PUMA∂ lack this domain. The BH3 domain is essential for binding of PUMAå and PUMA∫ to Bcl-2 or Bcl-xL. PUMA is an initiator of ©-radiation apoptosis and glucocorticoid-induced apoptosis in lymphoid cells in vivo. Bcl-2 family members generally regulate apoptosis and transmit death signals to mitochondria. Members of this family include both pro- and anti-apoptotic proteins that share homologous sequences known as Bcl-2 homology domains (BH1-4). The BH3 proteins, BID, NOXA, PUMA, NBK, Bim and Bad, are all pro-apoptotic and share sequence homology within the amphipathic alpha-helical BH3 region. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PUMAα and PUMAβ of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 57-193 mapping at the C-terminus of PUMA&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 57-193 mapping at the C-terminus of PUMAα of human origin |
| Entrez Gene | 27113 |
|---|
Application
Western blot analysis of PUMAα/β expression in etposide treated HeLa (A) and PMA treated A-431 (B) whole cell lysates.
Application
Immunofluorescence staining of normal mouse lymph node frozen section showing cytoplasmic and perinuclear staining.| Application Notes | recommended for detection of PUMAα and PUMAβ of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
