Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing cytoplasmic localization.Application
Western blot analysis of cHMGCS expression in non-transfected 293T: (A), mouse cHMGCS transfected 293T: (B) and Hep G2 (C) whole cell lysates.Application
Western blot analysis of cHMGCS expression in non-transfected: (A) and mouse cHMGCS transfected: (B) 293T whole cell lysates.Application
Western blot analysis of cHMGCS expression in non-transfected: (A) and human cHMGCS transfected: (B) 293T whole cell lysates.| Product Name | cHMGCS Antibody (H-70) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 381-450 mapping near the C-terminus of cHMGCS of human origin |
| Modification Notes | HMG-CoA Synthase exists as both a mitochondrial (mHMGCS) and cytoplasmic (cHMGCS) enzyme that condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA. The HMG-CoA produced by cHMGCS is transformed into mevalonate by HMG-CoA reductase, which starts isoprenoid biosynthesis. End products of the isoprenoid pathway include cholesterol, ubiquinone, dolichol, isopentenyl adenosine and farnesyl groups. mHMGCS, together with HMG-CoA Lyase, is responsible for ketone body biosynthesis. mHMGCS is expressed in liver and kidney. Fasting, cAMP and fatty acids increase the level of transcription of mHMGCS, while feeding and insulin repress it. A regulatory element within the mHMGCS promoter confers transcriptional regulation by PPAR, RXR, COUP-TF and HNF-4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of cytoplasmic HMG-CoA Synthase of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 381-450 mapping near the C-terminus of cHMGCS of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 381-450 mapping near the C-terminus of cHMGCS of human origin |
| Entrez Gene | 3157 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing cytoplasmic localization.
Application
Western blot analysis of cHMGCS expression in non-transfected 293T: (A), mouse cHMGCS transfected 293T: (B) and Hep G2 (C) whole cell lysates.
Application
Western blot analysis of cHMGCS expression in non-transfected: (A) and mouse cHMGCS transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of cHMGCS expression in non-transfected: (A) and human cHMGCS transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of cytoplasmic HMG-CoA Synthase of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
